Translational Incorporation of Dehydro Amino Acids into Proteins — ASN Events
  1. Schedule
  2. Session Ten - Poster Session C
  3. Translational Incorporation of Dehydro Amino Acids into Proteins

Translational Incorporation of Dehydro Amino Acids into Proteins (#340)

Samuel A Fraser 1 , James E Hennessy 1 , Dannon J Stigers 1 , Christopher J Easton 1
  1. Australian National University, Canberra, ACT, Australia

The occurrence of dehydro amino acid residues in proteins has been associated with various physiological conditions. It is commonly thought that dehydrogenation occurs through oxidation following protein synthesis, however it has been established this is not exclusively the case.1 

The other pathway to dehydro proteins is by incorporation of modified amino acids. Generally, misincorporation occurs when an amino acid is isosteric to one of the twenty amino acids and is charged onto a tRNA by the cognate aminoacyl tRNA synthetase. We have systematically investigated the incorporation of dehydro amino acid analogues of the aliphatic amino acids during cell-free protein synthesis and the effects on the structure and function of the resultant proteins. 

  1. (1) M.L. Mock, T. Michon, J. C. M. van Hest, D. Tirrell, ChemBioChem, 2006, 7, 83-87.