RNA is an essential intermediate in the interpretation of our genetic information and in many cases can independently regulate development and metabolism. We are only really beginning to understand the network of proteins that engage with these RNA species to mediate their activity. We have discovered an RNA-binding protein, ZRANB2, that is essential for early mammalian development and we hypothesize that it exerts this function by controlling the processing of specific RNA targets. In an effort to delineate the mechanism of action of this protein, we have used experimental approaches ranging from biophysics and structural biology (1) through to biochemical analysis (2), RNAi, CLIPseq and the global analysis of RNA transcripts from a ZRANB2 knockout mouse. This presentation will describe our efforts to integrate these diverse datasets to understand the role ZRANB2 plays in eukaryotic biology.