Biological SAXS at the Australian Synchrotron — ASN Events

Biological SAXS at the Australian Synchrotron (#439)

Haydyn D.T. Mertens 1 , Stephen Mudie 1 , Adrian Hawley 1 , Nathan Cowieson 1 , Vesna Samardzic-Boban 1 , Nigel Kirby 1
  1. Australian Synchrotron, Clay ton, VIC, Australia

Small-Angle X-ray scattering (SAXS) of biomolecules for the characterisation of structure and complex formation is now utilised by many research groups1 . New developments in the validation of results of scattering experiments as applied to biological systems and the recent adoption of guidelines for publishing these data2  have further increased user interest and confidence in the application of SAXS/SANS in biology. As a consequence, the generation of the highest quality scattering data from these weakly scattering systems is a major focus of SAXS beamlines at major facilities.  At the SAXS/WAXS beamline of the Australian Synchrotron the last year has seen a dramatic improvement in machine stability/reliability, the reduction of background and now the automation of data collection, data reduction and primary data analysis. Here we present our recent developments that facilitate high throughput data collection and analysis with the only limitation being sample stability/purity.

  1. Mertens & Svergun, J. Struct. Biol. (2010) 172, 128-141
  2. Jacques et al., Acta Cryst. (2012) D68, 620-626